I have been working on moving our site from 3.2.2 to 6.2.4, manually copying content. I have all of the content on the new site and the site is working and looking quite nicely. Now when I move the site from Windows Server 2003 IIS 6.0 to Windows 7 IIS 7.5 the site functions fine I can browse all of the content and all links are working, but when I try to login as host or admin I get an error in the UserLog In popup with this error.

Access is denied. Description: An error occurred while accessing the resources required to serve this request. The server may not be configured for access to the requested URL.

Error message 401.2.: Unauthorized: Logon failed due to server configuration. Verify that you have permission to view this directory or page based on the credentials you supplied and the authentication methods enabled on the Web server. Contact the Web server's administrator for additional assistance.

I believe I have all of permissions set properly on the Dnn622 folder, for example IIS AppPool/DefaultAppPool modify. I have searched this site and many others and some reference windows authetication but I am not using the AD features. Does anyone have any thoughts or ideas on this?

That was one of the most common suggestions so I hit that one right away. No improvement. I have noticed that on my site in IIS 7.5 if look at the basic settings  and Test Settings... I see that I have an Authorization warning in the results "Cannot verify access to path (C:\DnnDev)" and the details are telling me that I need make sure my appPool has the necessary rights to the folder, it does Not sure if that has any bearing on my issue or not but I thought it was worthy of a mention.

By the way Chris thanks for all of your work on the templates. I got my VS2012 development environment all set up and created my first module but need to get logged in to add it. That is how I discovered this issue.

Mark